BMT-53808 Methods in Single Cell Biology, 5 cr


Additional information about the course is provided at:
Suitable for postgraduate studies.


Andre Sanches Ribeiro


Toteutuskerta Periodi Vastuuhenkilö Suoritusvaatimukset
BMT-53808 2019-01 2 Andre Sanches Ribeiro
To pass the course, the student is required to:
a) Pass in all grading requisites.
b) Attend at least 80% of the lessons.
c) Pass the final assignment.

There is no final exam in this course.


The student will be introduced to a variety of experimental techniques and analysis tools in single cell, quantitative biology studies. The aim is to provide understanding of the techniques of single cell observation and on how to interpret the results. The focus of this course will be the manipulation and measurements of gene expression in prokaryotic systems using live cell multi-modal microscopy systems and flow cytometry. The model organism will be Escherichia coli. Finally, students will gain understanding of the objectives, applicability and limitations of the methods and techniques in quantitative single cell biology studies.


Sisältö Ydinsisältö Täydentävä tietämys Erityistietämys
1. The concept of cell to cell diversity     
2. Model organisms and constructs (plasmids and vectors), Gene transfer techniques     
3. Techniques for single-cell measurements of RNA and proteins      
4. Basics of Microscopy     
5. Basics of Flow Cytometry     
6. Basics of single-cell image analysis     
7. Project presentations     


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BMT-53808 Methods in Single Cell Biology, 5 cr BMT-53807 Methods in Single Cell Biology, 3 cr  

Päivittäjä: Sanches Ribeiro Andre, 15.08.2019