Acinetobacter baylyi ADP1 has awakened a lot of interest due to its high potential for metabolic studies and biotechnological applications, mainly for its interesting metabolic network and the compact easily-transformable genome. Single or multiple gene deletions and insertions can be carried out in a high through-put manner. A constraint-based metabolic model has been constructed for ADP1. We have investigated the performance and potentiality of A. baylyi for metabolic engineering applications by implementing several approaches to gene expression, including gene knock-outs by synthetic gene cassettes, recombinant gene expression, studies of native and non-native promoters, extracellular protein expression, fusion constructs, bioremediation potential, and gene expression in co-cultures.
Responsible persons: PhD Ville Santala, Professor Matti Karp
Researchers: MSc Tech Suvi Santala, BSc Tech Latifeh Azizi, BSc Tech Matti Kannisto, Research fellow Elena Efimova
Duration: 2008-