BioSHG
Biosensors based on second-harmonic generation
Principal Investigator at ASE: Prof. Jukka Lekkala
Duration of the project: during 2006 - 2008
Project description:
The target in the BioSHG project was to develop novel optical biosensors based on second harmonic generation (SHG) and molecular nanotechnology. SHG is a nonlinear optical process where a laser beam at frequency ω is applied on a material, and light at doubled frequency 2ω is generated. As any changes on material, caused e.g. by binding of bio-chemicals on surface, affect on SHG signal, it was expected that SHG could offer a very sensitive alternative for fluorescence and surface plasmon resonance (SPR) methods traditionally used to detect real time binding of bio-molecules in bio-interaction analysis and immunodiagnostics. Small and low cost fibre laser was intended to be used as laser in instrument prototype.
Results by the Sensor Technology and Biomeasurements group:
As the SHG and sample fabrication results by the partners and slower than expected development of fibre lasers did not support building originally planned instrument prototype, we focused on building a fully modifiable SPR device as reference setup for the SHG measurements done by professor Kauranen’s group. The device was used also to verify the successfulness and preservation of the samples produced by the VTT researchers. Due to the reduced tasks in the project, we used also some time on finding new applications for SPR, leading to one international conference paper, student laboratory work and some new projects proposals.
Continuation:
SPR device and knowhow were later utilized in PELOTON project.
Publications:
Niemi, T., Pessa, M., Kauranen, M., Lekkala, J., Kuittinen, M., Vikholm-Lundin, I., 2010. Nanophotonics. FinNano Final Seminar, small and huge, May 4 - 5, 2010, Helsinki, Finland.
Ryynänen, T., Lekkala, J., 2007. Backside detection of photoresist development endpoint using surface plasmon resonance, 18th Annual IEEE/SEMI® Advanced Semiconductor Manufacturing Conference (ASMC 2007), 11-12 June 2007, Stresa, Italy.
Niemi, T., Pessa, M., Kauranen, M., Lekkala, J., Kuittinen, M., Sadowski, J., Vikholm-Lundin, I., 2006. Nanophotonics – Exploring Optics in Nanoscale. FinNano Annual seminar 18.5.2006, Wanha satama, Helsinki, Finland.
Funding: NanoPhotonics (Tekes, FinNano programme), Teknologiakeskus Hermia Oy, Professia Oy, RefleKron Oy, Epicrystals Oy, Braggone Oy, DCA Instruments Oy, Agasonia Oy, KSV Instruments Oy, Wallac Oy, Heptagon Oy, Artic Diagnostics Oy
Personnel: Tomi Ryynänen, Markus Karjalainen and Jarkko Mäkiranta
Direct partners (BioSHG): TUT/Physics/Martti Kauranen and VTT/Inger Vikholm-Lundin
Other partners (NanoPhotonics): TUT/ORC and University of Joensuu
Measurement setup for simultaneous SHG and SPR detection.