Fluorescent protein toolbox for studying biological processes inside living cells
The discovery and development of fluorescent proteins during the past century has revolutionized the field of cellular and molecular biology. They are the backbone of the recent fluorescence microscopy and its application in life sciences. The invisible life of the cells became visible with the aid of this protein that enables direct visualization of the cellular process and dynamics in living cells in its normal physiological conditions.
“The answer for solving biological problems lies inside the living cell. The development of every fluorescent protein-based tool results in the deciphering of numerous intracellular processes. Therefore, it is very important to continue developing related tools to enable further understanding of cellular dynamics and biological processes,” Bobin George Abraham says.
George Abraham’s doctoral dissertation concerns the genetic engineering of fluorescent proteins by developing new tools for studying biological processes. He has developed novel fluorescent protein-based FRET (Fluorescence resonance energy transfer) pairs for fluorescence lifetime imaging microscopy studies. The redox sensor he has developed enables the monitoring of the intracellular redox status of live bacteria at a single-cell level. Furthermore, his study demonstrated the intramolecular FRET in fluorescent proteins and broadens the application of fluorescent protein in the development of novel in vitro biosensors.
Public defence of a doctoral dissertation on Friday, 4 December
MSc (Tech) Bobin George Abraham’s doctoral dissertation in the field of bioengineering entitled “Fluorescent Protein Toolbox: Protein Engineering Broadens the Range of in vitro and in vivo Applications of Fluorescent Proteins” will be publicly examined at the Faculty of Natural Sciences of Tampere University of Technology (TUT) in the Festia Pieni Sali 1 auditorium in the Festia building (address: Korkeakoulunkatu 6, Tampere, Finland) at 12 noon on Friday, 4 December 2015.
The opponent will be University lecturer, PhD Vesa Hytönen, University of Tampere, BioMediTech, and Professor Matti Karp from the Department of Chemistry and Bioengineering at TUT will act as Chairman.
Bobin George Abraham (31) comes from Kerala, India and works as a researcher at the Department of Chemistry and Bioengineering of TUT.
The dissertation is available online at: http://URN.fi/URN:ISBN:978-952-15-3655-7